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Journal of the Korean Academy of Rehabilitation Medicine 2001;25(4):653-665.
The Role of Nitric Oxide in Neuropathic Changes Induced by the Autograft of Nucleus Pulposus to the Sciatic Nerve in Rats.
Han, Tai Ryoon , Lee, Jong Min , Choi, Jong Chul , Kim, Jung Ran
1Department of Rehabilitation Medicine, Seoul National University College of Medicine.
2Department of Rehabilitation Medicine, Dongguk University College of Medicine.
3Department of Pathology, Dongguk University College of Medicine.
흰쥐의 좌골신경에서 수핵 자가이식에 의한 신경 병변과 Nitric Oxide의 역할
한태륜, 이종민1, 최종철1, 김정란2
서울대학교 의과대학 재활의학교실, 동국대학교 의과대학 1재활의학교실, 2병리학교실
Abstract

Objective
To investigate the neuropathic changes induced by nucleus pulposus and possible role of nitric oxide (NO) in the pathogenesis of painful radiculopathy.


Method
Autologous nucleus pulposus was harvested from the rat coccygeal intervertebral disc and grafted to the sciatic nerve. Pain behavior, neurophysiologic and pathologic changes were compared between autografted and sham operated group during 14-day-period. Western immunoblotting and immunohistochemistry with anti-nitrotyrosine mouse monoclonal antibody were used to compare the NO production and nerve damage in autografted and sham operated nerve tissues.


Results
Mechanical allodynia and thermal hyperalgesia were observed 2 days after autograft of nucleus pulposus and persisted during 14-day-period (p<0.05). Motor nerve conduction latency was delayed and compound muscle action potential amplitude was decreased 5 days after autograft (p<0.05). Histologically, nucleus pulposus induced severe inflammatory reaction with fibroblast proliferation and foamy macrophage infiltration, which were persisted during 14-day- period. More nitrated proteins were detected consistently in nerve tissues with autograft of nucleus pulposus and immunohistochemical staining of nitrotyrosine was prominent around foamy macrophages.


Conclusion
These data suggest that nucleus pulposus induce mechanical allodynia, thermal hyperalgesia and nerve dysfunction through inflammatory reaction with macrophage infiltration. NO and NO related tissue injury may play an important role in the pathogenesis of painful radiculopathy.

Key Words: Nitric oxide, Herniated intervertebral disc, Nucleus pulposus, Neuropathic pain


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