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Journal of the Korean Academy of Rehabilitation Medicine 2006;30(5):447-454.
Chronological Changes in Cerebral Infarction of Photochemical Thrombosis Model: Magnetic Resonance Imaging and Histopathological Correlation.
Moon, Seong Keun , Shin, Yong Il , Kim, Hyoung Ihl , Lee, Min Cheol , Jin, Chun Yan , Lee, Seoul , Yoon, Kwon Ha , Cai, Quan Yu , Chung, Gyung Ho
1Department of Neurosurgery, Wonkwang University Medical School, Korea.
2Department of Rehabilitation Medicine, Wonkwang University Medical School, Korea. cbrmshin@wonkwang.ac.kr
3Department of Pharmacology, Wonkwang University Medical School, Korea.
4Department of Institute for Radiological Imaging Science, Wonkwang University Medical School, Korea.
5Department of Neurosurgery, Presbyterian Medical Center, Korea.
6Department of Pathology, Chonnam National University School of Medicine, Korea.
7Department of Radiology, Chonbuk National University School of Medicine, Korea.
광화학적 색전모델 뇌경색의 경시적 변화: 자기공명영상과 조직학적 소견
문성근, 신용일1, 김형일2, 이민철3, 김춘연3, 이서울4, 윤권하5, 채권우5, 정경호6
원광대학교 의과대학 신경외과학교실, 1재활의학교실, 4약리학교실 및 5익산 방사선 영상과학 연구소, 2예수병원 신경외과 3전남대학교 의과대학 병리학교실, 6전북대학교 의과대학 진단방사선과학교실
Abstract
Objective
Authors investigated magnetic resonance imaging (MRI) and histological characteristics of photothrombotic infraction rat model (PIRM) on long term basis to provide a basis for further research.
Method
Photothrombotic ischemia was induced in male Sprague-Dawley rats using Rose-bengal dye (20 mg/kg) and cold light. MRI was performed 1, 6, 12, 24 hours, 3, 7 days, 2, 3, 4, 6, and 8 weeks after photothrombosis and obtained T1- & T2-weighted and contrast-enhanced images. Also, T2* images were obtained after superparamagnetic iron oxide injection. After MRI, animals were sacrificed and the brain sections were stained for routine immunohistopathology.
Results
MRI and histological analysis revealed well in-duced lesion in the cortex and showed biological course of infarction. However, PIRM showed rapid development of infarction lacking collateral circulation. Infarction size reached maximum 12 hours after induction, progressively decreasing over 4 weeks. Interstitial and cytotoxic edema were evident at 6, 12, 24 hours, but decreasing afterwards. Neurogenic inflammation appeared on 3rd day and reached maximum on 5∼7th day. Arachnoid membrane was characteristically invaded with inflammatory cells and later thickened with fibrosis.
Conclusion
This study showed PIRM is ideal model to study subacute and chronic stages of cerebral infarction. (J Korean Acad Rehab Med 2006; 30: 447-454)
Key Words: Cerebral infarction, Rat model, Photothrombosis, Magnetic resonance imaging, Histopathology
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