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Journal of the Korean Academy of Rehabilitation Medicine 2007;31(4):447-456.
Protein Expression Profile of Synovial Fibroblasts in Experimental Post-traumatic Arthritis.
Kim, Eun Kuk , Ahn, Kyung Hoi , Kim, Hee Sang , Lee, Jong Ha , Choi, Kung Duk
1Department of Rehabilitation Medicine, Kyung Hee University Medical Center, Kyung Hee University College of Medicine, Korea. lkimg@paran.com
2Genomic Information Center, Hankyong National Univeristy, Korea.
실험적 외상 후 관절염에서 윤활막 섬유모세포의 단백질 발현 분석
김은국, 안경회, 김희상, 이종하, 최강덕1
경희대학교 의과대학 재활의학교실, 1한경대학교 생명공학연구소
Abstract
Objective
To evaluate and compare protein expression profiles of synovial fibroblasts using proteome analysis in swine knee injuries with joint instability, during early post-traumatic arthritis (PTA) development.
Method
Experimental PTA was induced by transection of the anterior cruciate ligament (ACL) in swine left knee joints. After sacrifice at 8 weeks, cartilage and synovium obtained from both knee joints were prepared for histopathologic examination. Cultured synovial fibroblasts were processed for 2-dimensional electrophoresis and mass spectrometric analysis. Histopathologic examination showed overt arthritic changes that supported the development of early PTA.
Results
Proteome analyses led to the identification of more than 1,500 protein spots and of 11 differently expressed protein spots. Of those, six proteins were down-regulated (cytoskeletal β actin, cofilin-1, destrin, Rho GDP dissociation inhibitor α, and unnamed protein product), and five proteins were up-regulated (α-B crystallin, smooth muscle protein 22-α, and cytoskeletal β actin) in ACL-transected synovial fibroblasts. That is, proteins related to cellular organization and signal transduction are down-regulated, and those related to cell rescue, defence, and stress are up-regulated.
Conclusion
These results may suggest that joint instability contributes to the development of PTA and is one of the major etiologic factors of PTA. In addition, this suggests that the proteome analysis of synovial fibroblasts is a useful approach in examining a joint after an injury and can be used to understand the pathogenesis of PTA. (J Korean Acad Rehab Med 2007; 31: 447-456)
Key Words: Post-traumatic arthritis, Proteome analysis, Anterior cruciate ligament, Down-regulated, Up-regulated
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