| Behavioral Testing for Therapeutic Outcome Measurements in an Animal Model of Huntington's Disease. |
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Cho, Sung Rae , Park, Eun Sook , Kim, Hyun Ok , Kim, Dong Goo , Park, Chang Il |
1Department of Rehabilitation Medicine and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Korea. pes1234@yumc.yonsei.ac.kr
2Department of Laboratory Medicine, Yonsei University College of Medicine, Korea.
3Department of Pharmacology, Yonsei University College of Medicine, Korea. |
| 헌팅톤 병 동물 모델에서 치료효과 판정을 위한 운동기능 평가 |
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조성래, 박은숙, 김현옥1, 김동구2, 박창일 |
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연세대학교 의과대학 재활의학교실 및 재활의학연구소, 1진단검사의학교실, 2약리학교실 |
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| Abstract |
Objective
To investigate which outcome measurements are useful for detecting functional changes after therapeutic approach to delayed motor impairment in an animal model of Huntington's disease (HD).
Method
R6/2 transgenic mice received intraventricular injections of adenoviral BDNF/noggin (AdB/N), AdBDNF, AdNull (n=15 each) at 4 weeks of age. Untreated R6/2s and wild-type mice were also recruited as controls. Motor performance was measured using rotarod analysis and locomotor activity test at regular intervals until preterminal age of 13 weeks.
Results
On constant speed rotarod testing, AdB/N-treated R6/2s exhibited a delayed disease progression after post- operative 6 weeks. AdB/N also ameliorated general locomotor activity deficits. One min-rotarod analysis showed a delayed motor impairment in AdBDNF group at preterminal age compared with AdNull and untreated controls, which was not shown in 3 min and 5 min-rotarod. Accelerating rotarod paradigm was not superior to constant speed. Partial therapeutic effects on locomotor activities were detected in total 60 min-monitoring, but not in 30 min- or 10 min- monitoring.
Conclusion
Appropriate behavioral testing and outcome measurements should be selected to detect the treatment effect to slow functional deterioration in HD. (J Korean Acad Rehab Med 2007; 31: 503-511) |
| Key Words:
Huntington's disease, Rotarod testing, Locomotor activity, Outcome measurement |
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