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Journal of the Korean Academy of Rehabilitation Medicine 2009;33(5):514-519.
Changes of Apoptosis Related Factors in Atrophic Rat Skeletal Muscles after Denervation and Disuse.
Seo, Kyoung Ho , Seo, Kyung Mook , Lim, Jae Young
1Department of Rehabilitation Medicine, Seoul National University College of Medicine Seoul National University Bundang Hospital, Korea. drlim1@snu.ac.kr
2Department of Rehabilitation Medicine, Hanil General Hospital, Korea.
3Department of Rehabilitation Medicine, Chung-Ang University College of Medicine, Korea.
탈신경과 무용 후 흰쥐 골격근의 근위축에서 세포자멸사 관련인자의 변화
서경호1, 서경묵2, 임재영
서울대학교 의과대학 분당서울대학교병원 재활의학교실, 1한일병원 재활의학과, 2중앙대학교 의과대학 재활의학교실
Abstract
Objective
To investigate the changes of apoptosis related factors after denervation and disuse in skeletal muscles and to find out the role of apoptosis in atrophic process.
Method
30 male Sprague-Dawley rats, 8 weeks of age were treated with three different atrophy models (10 rats in each group); hind-limbs suspension (HS) as a disuse model, complete denervation by sciatic nerve transsection (CD) and partial denervation by sciatic nerve crushing (PD). At 2 weeks and 4 weeks after each experiment, both gastrocnemius were dissected and their weights were measured. Western blotting for BAX and Bcl-2 and TUNEL assay were used to assess the changes of apoptosis related factors in muscle cell.
Results
The muscle weight of PD and CD group decreased 38.6%, 65.6%, respectively, of intact side at 4 weeks after injuries. The loss of muscle weight in HS group was smaller than that of denervated muscles. CD group showed high expression of BAX (3.45±0.32→2.87±0.48) and Bcl-2 (3.63±0.40→3.33±0.50) at 2 and 4 weeks after denervation, but in PD group, BAX at 2 weeks significantly decreased at 4 weeks (3.40±0.55→2.13±0.25). In HS group, their expressions were slightly increased only immediate after 14 days suspension (BAX: 1.30±0.38 and Bcl-2: 1.29±0.28). CD group showed higher number of positive nuclei (27.6±8.8%) than PD group (10.4±5.3%) and HS group (4.4±1.6%) in TUNEL assay.
Conclusion
Unlike temporary increase of apoptosis related factors in disuse, increase of these factors are remarkable and persisted after denervation. Muscle apoptosis may be the major cause of muscle atrophy during degeneration process. (J Korean Acad Rehab Med 2009; 33: 514-519)
Key Words: Apoptosis, Muscle atrophy, Denervation, Disuse, Skeletal muscles
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