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Journal of the Korean Academy of Rehabilitation Medicine 2009;33(5):520-526.
Human Mesenchymal Stem Cells Derived from Bone Marrow in Traumatic Brain Injury of Rat Migrate to the Site of Injury.
Kang, Si Hyun , Chun, Min Ho , Kim, Sang Tae , Cho, Hee Jin
1Department of Physical Medicine & Rehabilitation, Chung-Ang University, College of Medicine, Korea.
2Department of Rehabilitation Medicine, Asan Medical Center, University of Ulsan, College of Medicine, Korea. mhchun@amc.seoul.kr
3NMR Laboratory, ASAN Institute of Life Sciences, University of Ulsan, College of Medicine, Korea.
외상성 뇌손상 쥐에서 인체 골수유래 줄기세포의 손상부위로의 이동
강시현, 전민호1, 김상태2, 조희진2
중앙대학교 의과대학 재활의학교실, 1울산대학교 의과대학 서울아산병원 재활의학교실, 2서울아산병원 아산생명과학연구소 자기공명연구실
To define the migration and differentiation of adult human mesenchymal stem cells (hMSCs) derived from bone marrow, and their effect on neurobehavioral and cognitive improvements, after traumatic brain injury (TBI) in rats.
Two days after TBI, 1×106 hMSCs were injected into the corpus callosum of fifteen rats, on the contralateral side of TBI. Eleven rats received sham-operation as a control group. Neurobehavioral and Barnes maze tests, and magnetic resonance spectroscopy (MRS) were performed on days 1 and 28 after TBI. Immunohistochemical staining was performed to evaluate distribution and differentiation of hMSCs on day 56.
After 28 days, scores on the neurobehavioral test, Barnes maze test, and magnetic resonance spectroscopy (MRS) were higher than on day 1 in both the stem-cell and control groups, but there were no between-group differences. On day 56, injected hMSCs stained positively with MAB- 1281 were distributed in ipsilateral corpus callosum, lesion boundary zone, parietal cortex, and thalamic area around the lateral ventricle.
hMSCs injected to the contralateral side of TBI survive and migrate to various areas of the ipsilateral hemisphere. We observed no neurobehavioral or cognitive improvements in test animals, indicating the need to adjust experimental methodologies including the development of appropriate tests to evaluate neurobehavioral and cognitive functions of rats. (J Korean Acad Rehab Med 2009; 33: 520-526)
Key Words: Stem cell transplantation, Traumatic brain injury, Neurobehavioral manifestation, Magnetic resonance spectroscopy, Rats
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