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Journal of the Korean Academy of Rehabilitation Medicine 2003;27(6):990-996.
Effect of Conditioned Ischemic Stresses on NF-kappaB Activation in Rat Skeletal Muscle.
Lee, Jeong Eun , Lee, Chyung Ki , Yoon, Tae Sik , Hah, Jong Sik
1Department of Rehabilitation Medicine, Ewha Womans University College of Medicine, Korea. ipray@hosanna.net
2Department of Physiology, Ewha Womans University College of Medicine, Korea.
흰쥐 골격근의 허혈성 스트레스 조건하에서 NF-κB 활성 변화
이정은, 이청기, 윤태식, 하종식1
이화여자대학교 의과대학 재활의학교실, 1생리학교실
Abstract
Objective
To investigate the effect of conditioned ischemic stresses on NF-κB activities and the protective role of genistein in rat skeletal muscle.


Method
Rectus femoris muscles of rats were divided into 4 groups according to conditioned ischemic stresses as followings: sham-operated control group; 2 hour ischemia group by clamping the proximal femoral vessel; ischemia- reperfusion group by 30 minutes of reperfusion following 2 hours of ischemia; ischemia-reperfusion-electrical nerve stimulation group in which current was applied at the femoral nerve during 30 min of reperfusion period to induce repetitive muscle contraction. Either genistein-5% dimethyl sulfoxide (DMSO) or DMSO was injected 2 hours before ischemic induction. NF-κB activities were analysed by electrophoretic mobility shift assay.


Results
NF-κB activities pretreated with DMSO were significantly increased in ischemia, ischemia-reperfusion and ischemia-reperfusion-electrical nerve stimulation group compared with those of control group and in ischemia- reperfusion-electrical nerve stimulation group compared with those of ischemia group. Muscle edema was increased in ischemia- reperfusion-electrical nerve stimulation group. But, NF-κB activities and muscle edema pretreated with genistein were attenuated in ischemia-reperfusion-electrical nerve stimulation group compared with those pretreated with DMSO alone.


Conclusion
NF-κB activities reflect the severity of ischemic stresses. Pretreatment with genistein can attenuate NF-κB activities and muscle edema in ischemic stress of rat skeletal muscle. (J Korean Acad Rehab Med 2003; 27: 990-996)

Key Words: NF-κB, Ischemic stress, Electrical stimulation, Genistein, Skeletal muscle


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