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Journal of the Korean Academy of Rehabilitation Medicine 2007;31(3):269-277.
The Effects of Human Adipose Tissue Derived Mesenchymal Stem Cells on Degenerative Change of Disc in Rabbit Model.
Kim, Sang Beom , Kwak, Hyun , Yoon, Kisung , Lee, Kyeong Woo , Park, Ji Hoon , Kwon, Yong Seok , Han, Jin Yeong , Jeong, Jin Sook , Lee, Jong Hwa
1Department of Physical Medicine and Rehabilitation, Dong-A University College of Medicine, Korea. rm0007@hanafos.com
2Department of Plastic and Reconstructive Surgery, Dong-A University College of Medicine, Korea.
3Department of Laboratory Medicine, Dong-A University College of Medicine, Korea.
4Department of Pathology, Dong-A University College of Medicine, Korea.
가토 추간판의 퇴행성 변화에 대한 인체 지방조직유래 중간엽 줄기세포의 효과
김상범, 곽현, 윤기성, 이경우, 박지훈1, 권용석1, 한진영2, 정진숙3, 이종화
동아대학교 의과대학 재활의학교실, 1성형외과학교실, 2진단검사의학교실, 3병리학교실
Abstract
Objective
To determine whether transplanted human adipose tissue derived stem cells (hATSCs) can survive and increase the amount of proteoglycans in degenerated intervertebral disc.
Method
Lumbar disc degeneration was induced in thirty New Zealand white rabbits by injection of chondroitinase ABC. After 2 weeks, hATSCs were transplanted in degenerated disc in hATSCs group. Control group received phosphate buffered saline. The histologic grading and height of disc were measured at 2, 4, and 8 weeks after transplantation. The viability of donor cells was identified by using β-Actin gene polymerase chain reaction (PCR).
Results
4 and 8 weeks after hATSCs transplantation, the histologic grading showed significantly high score in hATSCs group (p<0.05), but the amount of proteoglycans was not significantly different between the two groups. The change of disc height was not significantly increased in hATSCs group. In the β-Actin gene PCR analysis, positive signal in the hATSCs group was observed.
Conclusion
hATSCs transplantation may be useful in decelerating disc degeneration in experimental models and provide new hopes for treatment of degenerative disc disease in humans. (J Korean Acad Rehab Med 2007; 31: 269-277)
Key Words: Transplanted human adipose tissue derived stem cells, Disc degeneration, Rabbit
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