The Assessment of Functional Change in Ischemic Stroke Rat.

, Lim, Seong Hoon; , Lee, Jung Soo; , Lee, Be Na; , Min, Ji Hye; , Lee, Jong In; , Rhee, Won Ihl; , Ko, Young Jin; , Kim, Hye Won

Original Article

Journal of the Korean Academy of Rehabilitation Medicine 2008;32(3):253-257.

Lim, Seong Hoon , Lee, Jung Soo , Lee, Be Na , Min, Ji Hye , Lee, Jong In , Rhee, Won Ihl , Ko, Young Jin , Kim, Hye Won

Department of Rehabilitation Medicine, College of Medicine, The Catholic University of Korea, Korea. kimhw@catholic.ac.kr

뇌경색 백서의 기능적 변화 평가

임성훈, 이정수, 이베나, 민지혜, 이종인, 이원일, 고영진, 김혜원

가톨릭대학교 의과대학 재활의학교실

Abstract

Objective
To investigate and quantify functional deficit in ischemic stroke rat.
Method
Male Sprague-Dawley rats were divided into sham group and stroke group. Permanent cerebral ischemia was inflicted on the stroke group by occluding the middle cerebral artery (MCAO). The proportion of hind limb weight bearing pressure was also measured. The withdrawal threshold values in the hindpaws were measured by using von-Frey hair for seven days from the day before ischemia.
Results
The proportion of paretic hind limb pressure to whole hind limb pressure in stroke group was significantly reduced than sham group (p＜0.05). The withdrawal threshold values of the paretic hindpaw in stroke group were not different from those in the sham group during whole experimental session (p＞0.05). Garcia motor score of stroke group was reduced significantly than sham group in whole experimental session after MCAO (p＜0.05). The proportions of right hind limb pressure showed no correlation with Garcia motor score after MCAO (p＞0.05).
Conclusion
Ischemic stroke in rats can lead to the reduction of pressure at paretic hind limb. The motor test for pressure weight borne on the paretic leg may provide functional information in the future studies with ischemic stroke rat. (J Korean Acad Rehab Med 2008; 32: 253-257)

Key Words: Behavior, Functional deficit, Stroke, Middle cerebral artery occlusion, Rat