| Effects of Transplantation of Human Embryonic Stem Cellson Functional Recovery in Spinal Cord Injured Rats. |
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Jung, Kwang Ik , Park, Chang Il , Park, Eun Sook , Shin, Ji Cheol |
1Department of Physical Medicine and Rehabilitation, Hallym University College of Medicine, Korea. jki@hallym.ac.kr
2Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Korea. |
| 척수손상 쥐에서 인간 배아 줄기세포의 이식이 기능적 회복에 미치는 효과 |
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정광익, 박창일1, 박은숙1, 신지철1 |
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한림대학교 의과대학 재활의학교실, 1연세대학교 의과대학 재활의학교실 및 재활의학연구소 |
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| Abstract |
Objective
To investigate the functional recovery following the transplantation of human embryonic stem (hES) cells into an injured rat spinal cord.
Method
Sprague-Dawley rats were subjected to the spinal cord injury (SCI) using the New York University impactor. The rats were randomly allocated into three groups of 12 rats each, one media-treated and two hES cell-transplanted groups (5×103/5Ռl, 2×104/5Ռl). The hES cells were transplanted 1 week after a SCI.
Results
The hES cells transplanted into the rats were found to promote the hind limb performance 8 weeks after transplantation. In the electrophysiological study, the transplanted rats showed significantly shortened latencies and increased amplitudes of motor and somatosensory evoked potentials, compared to the media-treated rats. In the spinal cord of the hES cell-treated group, the pathological findings including the glial scar formation and degenerative changes were attenuated and the human Tau protein-positive cells were identified in the vicinity of the necrotic cavity and in the white matter.
Conclusion
These results suggest that the transplantation of hES cells might play a role in promoting the functional recovery after a SCI. (J Korean Acad Rehab Med 2008; 32: 491-500) |
| Key Words:
Spinal cord injury, Human embryonic stem cells, Transplantation, Functional recovery |
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