The Effect of Steroid on Heat Shock Protein 70 Expression in mdx Mice.

, Lim, Jeong Hoon; , Bang, Moon Suk

Original Article

Journal of the Korean Academy of Rehabilitation Medicine 2009;33(1):1-4.

¹Department of Rehabilitation Medicine, Konkuk University Medical Center, Korea. jhlim@kuh.ac.kr
²Department of Rehabilitation Medicine, Seoul National University College of Medicine, Korea.

근육병 모델에서 스테로이드가 HSP70 발현에 미치는 영향

임정훈, 방문석¹

건국대학교병원 재활의학과, ¹서울대학교 의과대학 재활의학교실

Abstract

Objective
To investigate the effect of steroid administration on the apoptosis and heat shock protein 70 (HSP70) expression after exercise in the animal model of Duchenne muscular dystrophy.
Method
We measured Bcl-2, BAX and HSP70 expression by western blotting. 20 control and 20 mdx mice were divided into free-living (n=10) and exercise (n=10) groups. Free-living and exercise groups were further divided into steroid-treated and sham-treated groups to evaluate the effect of steroid administration.
Results
Apoptosis was most prominent in the sham-treated exercise group, while apoptosis was significantly reduced in the steroid-treated exercise group. HSP70 expression was maximized in sham-treated exercise group, whereas steroid administration inhibited HSP70 expression after exercise in muscular dystrophy animal model. Exercise loading was found to cause severe apoptosis but steroid administration alleviated apoptotic damage in mdx mice. s
Conclusion
HSP70 expression was suppressed in the steroid-treated exercise group, which suggests steroid might have major preventive effect in exercise-induced apoptosis of muscular dystrophy animal model. (J Korean Acad Rehab Med 2009; 33: 1-4)

Key Words: mdx mouse, Steroid, Exercise, Heat shock protein