J Korean Acad Rehabil Med Search


Journal of the Korean Academy of Rehabilitation Medicine 2010;34(4):387-393.
Change of Tetrodotoxin Sensitive Inhibitory Neural System of the Colon in Spinal Cord Injured Rats.
Joo, Min Cheol , Lee, Seung Hoi , Oh, Jung Taek , Jo, Hyang Jeong , Park, Hyo In , Lim, Yu Hyun , Choi, Suck Chei , Lee, Moon Young
1Department of Rehabilitation Medicine, Wonkwang University School of Medicine, Korea.
2Department of Physiology and Institute of Wonkwang Medical Science, Wonkwang University School of Medicine, Korea. lmy6774@wku.ac.kr
3Department of Surgery, Wonkwang University School of Medicine, Korea.
4Department of Pathology, Wonkwang University School of Medicine, Korea.
5Department of Internal Medicine and Digestive Disease Research Institute, Wonkwang University School of Medicine, Korea.
척수 손상 흰쥐의 대장에서 테트로드톡신 민감성 억제 신경계의 변화
주민철, 이승회1, 오정택2, 조향정3, 박효인, 임유현, 최석채4, 이문영1
원광대학교 의과대학 재활의학교실, 1생리학교실 및 원광의과학연구소, 2외과학교실, 3병리학교실, 4내과학교실 및 소화기질환연구소
To investigate the effect of spinal cord injury (SCI) on contractions of whole colonic preparation isolated from rats under the inhibition of nitrergic inhibitory neural system using tetrodotoxin (TTX).
Twenty Sprague-Dawley rats were used. A complete spinal cord transection was performed surgically at the T10 cord level in spinal cord injured group. After 1 week of operation, sensory and motor functions were assessed and colon was removed under anesthesia for in vitro motility study. Whole colon was divided into four segments: proximal, two mid colon and distal colon. Each segment of colon was mounted with longitudinal direction in a 10 ml organ bath. After 1 hour of equilibration, frequency, area under the curve of spontaneous contraction and the response to acetylcholine (Ach), KCl and TTX were measured in each segment. Also the responses to Ach and KCl response under TTX pretreatment were measured.
Enhanced contractile response to KCl solution (40 mM), TTX (1 ՌM) and Ach (10−6 M) was observed in both group. There was no statistical difference in spontaneous, Ach and KCl induced contraction between control and SCI rats, but TTX induced contraction was decreased in SCI group than control group (p<0.05). In addtion, the Ach and KCl responses under the TTX pretreatment were significantly decreased in SCI group than control group (p<0.05).
These results suggest that the change of colonic contractility after the SCI is caused by at least partly from the change of TTX related inhibitory neural system. (J Korean Acad Rehab Med 2010; 34: 387-393)
Key Words: Spinal cord injury, Colon, Tetrodotoxin, Neural system


Browse all articles >

Terms of Use   |   Privacy Polity
Editorial Office
Department of Rehabilitation Medicine, Seoul National University Hospital
101 Daehak-ro, Jongno-gu, Seoul, Korea
Tel: +82-10-8678-2671    Fax: +82-2-6072-5244    E-mail: edit@e-arm.org; edit.karm@gmail.com
Business Registration: 110-82-07460                

Copyright © 2024 by Korean Academy of Rehabilitation Medicine.

Developed in M2PI

Close layer