Objective
To evaluate the efficacy of light-emitting diode (LED) and their dual-wavelengths as a treatment strategy for osteoarthritis.
Methods
We induced osteoarthritis in male Sprague-Dawley rats by intra-articular injection of sodium iodoacetate into the right rear knee joint. The animals with lesions were divided into an untreated group and an LED-treated group (n=7 each). In the LED-treated group, the lesioned knee was irradiated with lasers (850 and 940 nm) and dose (3.15 J/cm²) for 20 minutes per session, twice a week for 4 weeks. Knee joint tissues were stained and scanned using an in vivo micro-computed tomography (CT) scanner. Serum interleukin (IL)-6 and IL-18 levels were determined using enzyme-linked immuno-sorbent assay. Several functional tests (lines crossed, rotational movement, rearing, and latency to remain rotating rod) were performed 24 hours before LED treatment and at 7, 14, 21, and 28 days after treatment.
Results
LED-treated rats showed improved locomotor function and suppressed matrix-degrading cytokines. Micro-CT images indicated that LED therapy had a preserving effect on cartilage and cortical bone.
Conclusion
LED treatment using wavelengths of 850 and 940 nm resulted in significant functional, anatomical, and histologic improvements without adverse events in a rat model. Further research is required to determine the optimal wavelength, duration, and combination method, which will maximize treatment effectiveness.

Citations

• Effects of Dual Wavelength Photobiomodulation on Osseointegration in Grafted Areas
  Lucas de Sousa Goulart Pereira, Julia Raulino Lima, Elcio Marcantonio, Priscilla Barbosa Ferreira Soares, Suzane Cristina Pigossi, Guilherme José Pimentel Lopes de Oliveira
  Lasers in Surgery and Medicine.2026; 58(5): 429.     CrossRef
• BTEX-K Ameliorates Rheumatoid Arthritis Through Regulating the NF-κB and PPAR-γ Signaling Pathways in Incomplete Freund’s Adjuvant-Induced Arthritis Mice
  Joonpyo Hong, Jin-Ho Lee, Ga Young Lee, Jin-Hwan Oh, Hana Lee, Han Sung Kim, Tack-Joong Kim
  Biomedicines.2025; 13(7): 1524.     CrossRef
• Photobiomodulation Literature Watch December 2023
  James D. Carroll
  Photobiomodulation, Photomedicine, and Laser Surgery.2024; 42(10): 660.     CrossRef

Objective
To define the migration and differentiation of adult human mesenchymal stem cells (hMSCs) derived from bone marrow, and their effect on neurobehavioral and cognitive improvements, after traumatic brain injury (TBI) in rats. Method: Two days after TBI, 1×106 hMSCs were injected into the corpus callosum of fifteen rats, on the contralateral side of TBI. Eleven rats received sham-operation as a control group. Neurobehavioral and Barnes maze tests, and magnetic resonance spectroscopy (MRS) were performed on days 1 and 28 after TBI. Immunohistochemical staining was performed to evaluate distribution and differentiation of hMSCs on day 56. Results: After 28 days, scores on the neurobehavioral test, Barnes maze test, and magnetic resonance spectroscopy (MRS) were higher than on day 1 in both the stem-cell and control groups, but there were no between-group differences. On day 56, injected hMSCs stained positively with MAB- 1281 were distributed in ipsilateral corpus callosum, lesion boundary zone, parietal cortex, and thalamic area around the lateral ventricle. Conclusion: hMSCs injected to the contralateral side of TBI survive and migrate to various areas of the ipsilateral hemisphere. We observed no neurobehavioral or cognitive improvements in test animals, indicating the need to adjust experimental methodologies including the development of appropriate tests to evaluate neurobehavioral and cognitive functions of rats. (J Korean Acad Rehab Med 2009; 33: 520-526)

Objective
This study was evaluated the bone protective effects of the salmon calcitonin microspheres against the cancellous bone loss in the ovariectomized rats.

Method: The experimented rats were total 50 Sprague- Dawley female rats. They were divided randomly 5 groups. The treatment was initiated on the first day after surgery and continued for a period of 4 weeks. Bone measurements were performed in the distal femoral metaphysis and 5th lumbar vertebrae with dual energy x-ray absorptiometry at the time of 2nd week and 4th week after drug injection in all groups.

Results: The cancellous bone density in the ovariectomized rats treated with high dose of the salmon calcitonin microspheres was significantly higher than that of the free salmon calcitonin-treated ovariectomized rats, but less than that of the sham-operated control rats.

Conclusion: This study shows that the salmon calcitonin microspheres were evaluated for protection against the cancellous bone loss in the ovariectomized rats. The bone protective effect of the salmon calcitonin microspheres was greater than that of the free salmon calcitonin. (J Korean Acad Rehab Med 2003; 27: 121-125)

Objective
To investigate the changes of motor and soma-tosensory evoked potentials found in focal cerebral cortical ischemia induced by endothelin-1 (ET-1), one of the common models of cerebral infarct in rats. Method: A total of twenty Sprague-Dawley rats were studied. Focal cerebral cortical ischemia was induced by steterotaxic injection of ET-1 into forelimb region of cerebral cortex. Pellet retrieval test, motor evoked potential (MEP), and somatosensory evoked potential (SEP) were compared before and after cerebral ischemia. The location and extent of cerebral ischemia were confirmed histologi-cally. Results: Success rate of pellet retrieval test decreased significantly after induction of cerebral ischemia, demon-strating sensorimotor deficit in the contralateral forelimb. The latency and amplitude of MEP did not changed significantly despite weakness of forelimb. However, SEP showed reversal of the positive peaks. Conclusion: The results suggest that the changes of MEP and SEP in focal cerebral cortical ischemia are different from those in cerebral ischemia by large artery occlusion. When evaluating MEP and SEP in focal cerebral ischemia model, interpretation of evoked potentials should be cautious. (J Korean Acad Rehab Med 2009; 33: 147-153)